Anti-Mouse CD16/CD32 Antibody (2.4G2) (MP369107)

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Overview

Catalog No.	MP369107
Description	Anti-Mouse CD16/CD32 Antibody (2.4G2) [2.4G2] (MP369107) is a rat monoclonal antibody detecting CD16 in FCM, FuncS, IF, IP. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	FCM, FuncS, IF, IP
Host species	Rat
Isotype	IgG2b, kappa
Clone ID	2.4G2
Clonality	Monoclonal
Target	Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17
Endotoxin level	<1 EU/mg
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P08508, P08101
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Low affinity immunoglobulin gamma Fc region receptor III (CD16/CD32) is a ~30 kDa protein. Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b. Mediates neutrophil activation by IgG complexes redundantly with Fcgr4. 1. Hirano, M. et al. (2007) Nature immunology 8, 762-71. PMID: 17558411 2. Jakus, Z. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 618-29. PMID: 18097064
Note	For research use only.

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Bioactivity

Detects CD16/CD32 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-mouse CD16 antibody.CD16 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD16 antibody monoclonal antibody (Catalog # MP369107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Mouse CD16/CD32 Antibody (2.4G2).pdf

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Anti-Mouse CD16/CD32 Antibody (2.4G2) (MP369107)

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