FAQs

UniProt is a comprehensive protein database collaboratively maintained by EMBL-EBI, SIB, and NCBI. It comprises UniProtKB, Proteomes, UniRef, and UniParc, offering extensive protein sequences, functional annotations, and structural data. With integrated tools like BLAST and Align, UniProt supports advanced research in bioinformatics, molecular biology, and proteomics.

Learn how to choose the best isotype control antibody to ensure accurate in vivo research. Explore essential selection criteria, their role in experimental validation, and Abinscience’s high-quality, low-endotoxin antibody solutions.

Learn about polyethylene glycol (PEG), its properties, uses, and detection using anti-PEG antibodies. Find answers to common questions on PEG-related products.

Clone numbers identify specific hybridoma cell lines in monoclonal antibody production, ensuring consistency and traceability. Find them in the 'overview' tab of the product datasheet.

This guide helps researchers choose the most suitable internal control antibodies for Western Blot experiments. It outlines five key selection principles based on species, molecular weight, tissue type, expression stability, and experimental context. Practical tips and common pitfalls are included to ensure accurate and reliable protein normalization.

This article provides a comprehensive guide to troubleshooting common Western Blot (WB) issues, including high background, missing or blurry bands, and non-specific signals. Practical tips and optimization strategies are included to help researchers improve signal clarity, reduce noise, and ensure reliable results in protein detection experiments.

Protein quantification is vital in biochemistry, medicine, and food science for diagnostics and quality control. Methods like Lowry, BCA, UV spectrophotometry, and Kjeldahl offer unique approaches to measure protein concentration, each with specific principles, applications, and precautions. These techniques ensure accurate and reliable results for diverse samples, from serum to food, supporting research and industry needs.

This Western Blot protocol offers a detailed guide for protein detection, covering sample preparation, SDS-PAGE, and both indirect and direct immunoblotting methods. Ideal for molecular biology research, it ensures reliable results with abinScience’s high-quality antibodies.