Anti-Mouse CD16/CD32 Antibody (2.4G2)
Overview
| Catalog No. |
MP369107 |
| Host species |
Rat |
| Species reactivity |
Mouse |
| Form |
Liquid |
| Storage buffer |
0.01M PBS pH 7.4 |
| Purity |
>95% as determined by SDS-PAGE. |
| Clonality |
Monoclonal |
| Isotype |
IgG2b, kappa |
| Applications |
FCM, FuncS, IF, IP |
| Target |
Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17 |
| Purification |
Protein A/G purified from cell culture supernatant. |
| Endotoxin level |
<1 EU/mg |
| Accession |
P08508, P08101 |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Reconstitution |
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Clone ID |
2.4G2 |
| Shipping |
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note |
For research use only. |
Images
 Bioactivity |
Detects CD16/CD32 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-mouse CD16 antibody.CD16 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD16 antibody monoclonal antibody (Catalog # MP369107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer. |