Recombinant Human CD93/C1qR Protein, C-His (HV345011) expressed in Mammalian Cells, spanning Met1-Lys580.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian Cells
Accession
Q9NPY3
Protein length
Met1-Lys580
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Predicted molecular weight
61.35 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Complement component C1q receptor (CD93/C1qR) is a ~68 kDa protein. Cell surface receptor that plays a role in various physiological processes including inflammation, phagocytosis, and cell adhesion. Plays a role in phagocytosis and enhances the uptake of apoptotic cells and immune complexes by acting as a receptor for defense collagens including surfactant protein A/SFTPA1, C1q, and mannose-binding lectin (MBL2). Plays a role in the regulation of endothelial cell function and adhesion by activating angiogenesis. Mechanistically, exerts its angiogenic function by associating with beta-dystroglycan, leading to SRC-dependent phosphorylation and subsequent recruitment of CBL. In turn, CBL provides a docking site for downstream signaling components, such as CRKL to enhance cell migration.
1. Geertsma, MF. et al. (1994) The American journal of physiology 267, L578-84. PMID: 7977768
2. Orlandini, M. et al. (2014) Oncotarget 5, 2750-60. PMID: 24809468
3. Galvagni, F. et al. (2016) Oncotarget 7, 10090-103. PMID: 26848865
4. Khan, KA. et al. (2017) Oncogene 36, 6097-6108. PMID: 28671670
5. Barbera, S. et al. (2023) International journal of biological macromolecules 224, 453-464. PMID: 36265539
6. Xu, Y. et al. (2024) Structure (London, England : 1993) 32, 282-291.e4. PMID: 38218180
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD93/C1qR Protein, C-His
Bioactivity
Detects Anti-Human CD93/C1qR(070-016) in indirect ELISAs.
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