Anti-Human CD24 Antibody (SWA11) (HB630107)

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Datasheet

Overview

Catalog No.	HB630107
Description	Anti-Human CD24 Antibody (SWA11) [SWA11] (HB630107) is a mouse monoclonal antibody detecting CD24 in FCM, IHC, IF, RIA. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM, IHC, IF, RIA
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	SWA11
Clonality	Monoclonal
Target	Signal transducer CD24, CD24, CD24A, Small cell lung carcinoma cluster 4 antigen
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P25063
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Signal transducer CD24 is a ~8 kDa protein. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. 1. Suzuki, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5567-77. PMID: 11313396
Note	For research use only.

Images

SEC-HPLC

SEC-HPLC detection for Anti-Human CD24 Antibody (SWA11).
Bioactivity

Detects CD24 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human CD24 Antibody (SWA11)
Flow Cytometry

Flow-cytometry using anti-human CD24 antibody. Human SK-BR-3 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD24 antibody monoclonal antibody (Catalog # HB630107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD24 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD24 (Catalog No: HB630011)
Lane 2: negative control