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Recombinant Human CD24 Protein, C-Fc (HB630011)

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Recombinant Human CD24 Protein, C-Fc

Recombinant Human CD24 Protein, C-Fc

Recombinant Human CD24 Protein, C-Fc

Recombinant Human CD24 Protein, C-Fc

Recombinant Human CD24 Protein, C-Fc

Recombinant Human CD24 Protein, C-Fc

Recombinant Human CD24 Protein, C-Fc

Recombinant Human CD24 Protein, C-Fc

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Overview
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References
Datasheet

Overview

Catalog No.	HB630011
Description	Recombinant Human CD24 Protein, C-Fc (HB630011) expressed in Mammalian Cells, spanning Met1-Ala57. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P25063
Protein length	Met1-Ala57
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	32.18 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Signal transducer CD24, CD24, CD24A, Small cell lung carcinoma cluster 4 antigen
Background	Signal transducer CD24 is a ~8 kDa protein. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. 1. Suzuki, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5567-77. PMID: 11313396
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD24 Protein, C-Fc
Bioactivity

Detects Human CD24 Antibody (3B6) in indirect ELISAs.
Bioactivity

Detects Human CD24 Antibody (SWA11) in indirect ELISAs.
Western blot

Detected by Anti-CD24 monoclonal antibody (Catalog No: HB630107)

References

HB630011-SDS-PAGE-1.jpg

Datasheet

Recombinant Human CD24 Protein, C-Fc.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

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Working Solution

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Recombinant Human CD24 Protein, C-Fc