| Catalog No. | BY380012 |
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| Description | Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient. |
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| Protein length | Terminal Transferase (TdT) is expressed in E. coli. |
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| Applications | Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. |
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| Species | Bovine |
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| Nature |
Recombinant |
| Endotoxin level |
Please contact with the lab for this information. |
| Purity |
>90% as determined by SDS-PAGE. |
| Predicted molecular weight |
45 kDa |
| Form |
Liquid |
| Storage buffer |
100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| Shipping |
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Alternate Names | DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT |
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| Note |
For research use only. |
