Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient.
Terminal Transferase (TdT)
Overview
Catalog No.
BY380012
Form
Liquid
Storage buffer
100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol.
Purity
>90% as determined by SDS-PAGE.
Applications
Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
Endotoxin level
Please contact with the lab for this information.
Protein length
Terminal Transferase (TdT) is expressed in E. coli.
Nature
Recombinant
Predicted molecular weight
45 kDa
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Alternative name
DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT
Species
Bovine
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Note
For research use only.
Description
Images
SDS-PAGE
SDS-PAGE for Terminal Transferase (TdT)