Anti-HPV16 E6/Protein E6 Polyclonal Antibody (VK451024)

Datasheet Share product Copy info

Price(USD): $

Spec:

20ug
50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK451024

Description

Anti-HPV16 E6/Protein E6 Polyclonal Antibody (VK451024) is a rabbit polyclonal antibody detecting HPV16 E6/Protein E6 in WB, IHC, ELISA. Suitable for HPV16.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

HPV16

Applications

WB,IHC,ELISA

Host species

Rabbit

Isotype

IgG

Species

Human papillomavirus type 16 (Human)

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HPV16 E6/Protein E6 (Met1-Leu158).

Target

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P03126

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Protein E6, E6

Background

Protein E6 is a ~19 kDa protein. Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host UBE3A/E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6AP targets several other substrates to degradation via the proteasome including host DLG1 or NFX1, a repressor of human telomerase reverse transcriptase (hTERT).

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HPV16 E6/Protein E6 polyclonal antibody at 1ug/mL on recombinant HPV16 E6/Protein E6 (Catalog No. VK451012).

References

Datasheet