Anti-Lassa virus/LASV GPC Polyclonal Antibody (VK453024)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

VK453024

Description

Anti-Lassa virus/LASV GPC Polyclonal Antibody (VK453024) is a rabbit polyclonal antibody detecting GPC in ELISA, IHC, WB. Suitable for Lassa virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Lassa virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Lassa virus/LASV GPC (Gly260-Asp432).

Target

Pre-glycoprotein polyprotein GP complex, Pre-GP-C, Stable signal peptide, SSP, Glycoprotein G1, GP1, Glycoprotein G2, GP2, GPC, GP-C

Purification

Purified by antigen affinity column.

Accession

P08669

RRID

Anti-Lassa virus/LASV GPC Polyclonal Antibody (abinScience Cat# VK453024, RRID:AB_3730460)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pre-glycoprotein polyprotein GP complex (GPC) is a ~55 kDa protein. Functions as a cleaved signal peptide that is retained as the third component of the GP complex (GP-C). Helps to stabilize the spike complex in its native conformation. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of G1 and G2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.

1. Katz, M. et al. (2022) Nature 603, 174-179. PMID: 35173332

2. Eichler, R. et al. (2003) EMBO reports 4, 1084-8. PMID: 14555961

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Lassa virus/LASV GPC polyclonal antibody at 1ug/mL on recombinant Lassa virus/LASV GPC (Catalog No. VK453022).
Western blot

Western blot analysis was performed using anti-RSV-GP2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Lassa virus/LASV GPC (Catalog No: VK453022)