Research Grade Anti-Human CD73/NT5E (IBI-325) (HB209186)

Datasheet Share product Copy info

Price(USD): $

Spec:

100ug
1mg

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HB209186

Description

Anti-Human CD73/NT5E (IBI-325) (HB209186) is a research-grade recombinant antibody targeting CD73.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.

Species reactivity

Human

Applications

ELISA, FACS, Functional assay

Host species

Human

Isotype

IgG4, kappa (F234A, L234A)

Clone ID

IBI-325

Clonality

Monoclonal

Target

5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P21589

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152

2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138

3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347

4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587

5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD73/NT5E (IBI-325)

References

Datasheet

Research Grade Anti-Human CD73/NT5E (IBI-325).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Research Grade Anti-Human CD73/NT5E (IBI-325) (HB209186)

Overview

Images

References

Datasheet

Quick access

Contact Us