Resaerch Grade Anti-EGFRvIII Antibody (AMG-595) (HF004516)

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1mg

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Overview

Catalog No.	HF004516
Description	Resaerch Grade Anti-EGFRvIII Antibody (AMG-595) [AMG-595] (HF004516) is a human monoclonal antibody detecting EC:2.7.10.1 in ELISA, FACS, Functional assay. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	ELISA, FACS, Functional assay
Host species	Human
Isotype	IgG1, kappa
Clone ID	AMG-595
Clonality	Monoclonal
Target	EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Endotoxin level	< 10 EU/mg
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P00533
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Epidermal growth factor receptor (EGFRvIII) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. 1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725 2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536 3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960 4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033 5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049 6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079 7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029 8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704
Note	For research use only.

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SDS-PAGE for Resaerch Grade Anti-EGFRvIII Antibody (AMG-595)

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Datasheet

Resaerch Grade Anti-EGFRvIII Antibody (AMG-595).pdf

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Resaerch Grade Anti-EGFRvIII Antibody (AMG-595) (HF004516)

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