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Recombinant Human S100A8 Protein, N-Strep (HY242022)

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Recombinant Human S100A8 Protein, N-Strep

Recombinant Human S100A8 Protein, N-Strep

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Datasheet

Overview

Catalog No.	HY242022
Description	Recombinant Human S100A8 Protein, N-Strep (HY242022) expressed in E. coli, spanning Leu2-Glu93. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P05109
Protein length	Leu2-Glu93
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	12.16 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	S100 calcium-binding protein A8, Urinary stone protein band A, MRP8, Cystic fibrosis antigen, Calprotectin L1L subunit, MRP-8, p8, Calgranulin-A, Migration inhibitory factor-related protein 8, Leukocyte L1 complex light chain, S100A8, Protein S100-A8, CAGA, CFAG
Background	Protein S100-A8 (S100A8) is a ~10 kDa protein. S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69. 1. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human S100A8 Protein, N-Strep

References

HY242022-SDS-PAGE-1.jpg

Datasheet

Recombinant Human S100A8 Protein, N-Strep.pdf

Formula

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Recombinant Human S100A8 Protein, N-Strep