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Human CD269/TNFRSF17 Recombinant Protein (C-Fc) (HX959011)

Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
Human CD269/TNFRSF17 Recombinant Protein (C-Fc)
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Overview
Catalog No.HX959011
Description
Recombinant Human CD269/TNFRSF17 Protein, C-Fc (HX959011) expressed in Mammalian Cells, spanning Met1-Ala54. Purity: >90% by SDS-PAGE.
Highlights
  • Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
  • Mammalian Expression — Proper folding and native glycosylation.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemMammalian Cells
AccessionQ02223
Protein lengthMet1-Ala54
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesTumor necrosis factor receptor superfamily member 17, TNFRSF17, CD269, B-cell maturation protein, BCMA, BCM
Background

Tumor necrosis factor receptor superfamily member 17 (CD269/BCMA) is a ~20 kDa protein. Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. CD269 is the therapeutic target of teclistamab (Tecvayli).

Note For research use only.
Images
  • Human CD269/TNFRSF17 Recombinant Protein (C-Fc)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CD269/TNFRSF17 Protein, C-Fc

  • Human CD269/TNFRSF17 Recombinant Protein (C-Fc)

    Bioactivity

    Detects Belantamab in indirect ELISAs.

  • Human CD269/TNFRSF17 Recombinant Protein (C-Fc)

    Western blot

    Detected by Anti-CD269/TNFRSF17 monoclonal antibody (Catalog No: HX959016)

  • Human CD269/TNFRSF17 Recombinant Protein (C-Fc)

    Western blot

    Detected by Anti-CD269/TNFRSF17 monoclonal antibody (Catalog No: HX959026)

Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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