Anti-SOD1 Polyclonal Antibody (HF876014)

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Overview
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Overview

Catalog No.

HF876014

Description

Anti-SOD1 Polyclonal Antibody (HF876014) is a rabbit polyclonal antibody detecting SOD1 in ELISA, IHC, WB. Suitable for Human, Callithrix jacchus, Camelus dromedarius, Cavia porcellus, and Hylobates lar.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Callithrix jacchus, Camelus dromedarius, Cavia porcellus, Hylobates lar, Macaca fascicularis, Macaca fuscata fuscata, Macaca mulatta, Pan troglodytes, Pongo pygmaeus, Sapajus apella

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SOD1 (Met1-Gln154).

Target

Superoxide dismutase [Cu-Zn], SOD1, hSod1, Superoxide dismutase 1

Purification

Purified by antigen affinity column.

Accession

P00441

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Superoxide dismutase [Cu-Zn] (SOD1) is a ~15 kDa protein. Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides.

1. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062

2. Switzer, CH. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2205044120. PMID: 36630448

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SOD1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lane 4: K562 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: MCF-7 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Rat brain lysate
Lane 10: Rat liver lysate