Anti-JEV Envelope protein E Polyclonal Antibody (VK731014)

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50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

VK731014

Description

Anti-JEV Envelope protein E Polyclonal Antibody (VK731014) is a rabbit polyclonal antibody detecting Envelope protein E in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Japanese encephalitis virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Japanese encephalitis virus (JEV) Envelope protein E (Phe295-Ala700).

Target

Genome polyprotein, Envelope protein E

Purification

Purified by antigen affinity column.

Accession

P32886

RRID

Anti-JEV Envelope protein E Polyclonal Antibody (abinScience Cat# VK731014, RRID:AB_3725154)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (Envelope protein E) is a ~380 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-JEV Envelope protein E polyclonal antibody at 1ug/mL on recombinant JEV Envelope protein E (Catalog No. VK731022).
Western blot

Western blot analysis was performed using anti-JEV-Envelope protein E polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant JEV Envelope protein E (Catalog No: VK731022)