Anti-IL17F Polyclonal Antibody (HV544014)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HV544014

Description

Anti-IL17F Polyclonal Antibody (HV544014) is a rabbit polyclonal antibody detecting IL17F in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IL17F (Arg31-Gln163).

Target

IL17F, Interleukin-17F, IL-17F, Cytokine ML-1

Purification

Purified by antigen affinity column.

Accession

Q96PD4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-17F (IL17F) is a ~18 kDa protein. Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. IL17A-IL17F signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter through SEFIR domains. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. IL17A-IL17F is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation. As signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites.

1. Puel, A. et al. (2011) Science (New York, N.Y.) 332, 65-8. PMID: 21350122

2. Hymowitz, SG. et al. (2001) The EMBO journal 20, 5332-41. PMID: 11574464

3. Starnes, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 4137-40. PMID: 11591732

4. Kawaguchi, M. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 4430-5. PMID: 11591768

5. Kuestner, RE. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 5462-73. PMID: 17911633

6. Wright, JF. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 2799-805. PMID: 18684971

7. Goepfert, A. et al. (2017) Scientific reports 7, 8906. PMID: 28827714

8. Goepfert, A. et al. (2020) Immunity 52, 499-512.e5. PMID: 32187518

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IL17F polyclonal antibody at 1ug/mL on recombinant IL17F (Catalog No. HV544012).

References

Datasheet

Anti-IL17F Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-IL17F Polyclonal Antibody (HV544014)

Overview

Images

References

Datasheet

Quick access

Contact Us