Anti-Human TFPI Recombinant Antibody (SAA2013) (HY403013)

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Datasheet

Overview

Catalog No.

HY403013

Description

Anti-Human TFPI Antibody (SAA2013) [SAA2013] (HY403013) is a human monoclonal antibody detecting TFPI in ELISA, FCM, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, WB

Host species

Human

Isotype

IgG4, kappa

Clone ID

SAA2013

Clonality

Monoclonal

Target

Extrinsic pathway inhibitor, Lipoprotein-associated coagulation inhibitor, TFPI, EPI, LACI, TFPI1, Tissue factor pathway inhibitor

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P10646

RRID

Anti-Human TFPI Antibody (SAA2013) (abinScience Cat# HY403013, RRID:AB_3727925)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Tissue factor pathway inhibitor (TFPI) is a ~35 kDa protein. Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human TFPI antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TFPI antibody monoclonal antibody (Catalog # HY403013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human TFPI Antibody (SAA2013).

References

Datasheet

Anti-Human TFPI Recombinant Antibody (SAA2013).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TFPI Recombinant Antibody (SAA2013) (HY403013)

Overview

Images

References

Datasheet

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