Anti-Human SLC18A2 Polyclonal Antibody (HC560014)

Name: Anti-Human SLC18A2 Polyclonal Antibody
Brand: abinScience
SKU: HC560014
Price: 96 USD
Availability: InStock

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100ug

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Overview
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References
Datasheet

Overview

Catalog No.

HC560014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SLC18A2 (Pro42-Val129).

Target

SVMT, VMAT2, Monoamine transporter, Synaptic vesicular amine transporter, VAT2, SLC18A2, Solute carrier family 18 member 2, Vesicular amine transporter 2

Purification

Purified by antigen affinity column.

Accession

Q05940

RRID

Anti-Human SLC18A2 Polyclonal Antibody (abinScience Cat# HC560014, RRID:AB_3723262)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Synaptic vesicular amine transporter (SLC18A2/VMAT2) is a ~55 kDa protein. Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin. Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals.

1. Rilstone, JJ. et al. (2013) The New England journal of medicine 368, 543-50. PMID: 23363473

2. Pidathala, S. et al. (2023) Nature 623, 1086-1092. PMID: 37914936

3. Wu, D. et al. (2024) Nature 626, 427-434. PMID: 38081299

4. Dalton, MP. et al. (2024) eLife 12. PMID: 38517752

5. Erickson, JD. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 5166-71. PMID: 8643547

6. Erickson, JD. et al. (1995) Journal of molecular neuroscience : MN 6, 277-87. PMID: 8860238

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human SLC18A2 polyclonal antibody at 1ug/mL on recombinant Human SLC18A2 (Catalog No. HC560012).
Western blot

Western blot analysis was performed using anti-SLC18A2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate