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Anti-Human CD80/B7-1 Antibody (SAA2207), APC (HW630237)

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Anti-Human CD80/B7-1 Antibody (SAA2207), APC

Anti-Human CD80/B7-1 Antibody (SAA2207), APC

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Datasheet

Overview

Catalog No.	HW630237
Description	Anti-Human CD80/B7-1 Antibody (SAA2207), APC [SAA2207] (HW630237) is a human monoclonal antibody detecting CD80 in ELISA, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	SAA2207
Conjugation	APC
Clonality	Monoclonal
Target	CD80, BB1, T-lymphocyte activation antigen CD80, CD28LG, B7, CD28LG1, LAB7, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1
Endotoxin level	Please contact with the lab for this information.
Purification	Affinity chromatography.
Accession	P33681
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	T-lymphocyte activation antigen CD80 (B7-1) is a ~33 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. 1. Lu, B. et al. (2024) Acta pharmacologica Sinica 45, 1214-1223. PMID: 38467718 2. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 3. Boulougouris, G. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 1809-16. PMID: 10438913 4. Vandenborre, K. et al. (1999) Immunology 98, 413-21. PMID: 10583602 5. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502 6. Frauwirth, KA. et al. (2002) Immunity 16, 769-77. PMID: 12121659 7. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow Cytometry

Flow-cytometry using APC anti-Human CD80 antibody. Raji cells were stained with an irrelevant antibody (Red Histogram) or an APC anti-Human CD80 antibody monoclonal antibody (Catalog # HW630237 , Green Histogram at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD80/B7-1 Antibody (SAA2207), APC.pdf

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Anti-Human CD80/B7-1 Antibody (SAA2207), APC