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Anti-Human CA9 Recombinant Antibody (SAA0152) (HS840107)

Anti-Human CA9 Recombinant Antibody (SAA0152)
Anti-Human CA9 Recombinant Antibody (SAA0152)
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  • Datasheet

Overview
Catalog No.HS840107
Description
Anti-Human CA9 Antibody (SAA0152) [SAA0152] (HS840107) is a human monoclonal antibody detecting CA9 in FCM. Suitable for Human.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
Species reactivityHuman
ApplicationsFCM
Host speciesHuman
IsotypeIgG1, kappa
Clone IDSAA0152
Clonality Monoclonal
Target Membrane antigen MN, P54/58N, Carbonic anhydrase 9, Renal cell carcinoma-associated antigen G250, CA-IX, CAIX, pMW1, Carbonic anhydrase IX, RCC-associated antigen G250, Carbonate dehydratase IX, CA9, MN, G250
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q16790
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Carbonic anhydrase 9 (CA9) is a ~49 kDa protein. Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

Note For research use only.
Images
  • Anti-Human CA9 Recombinant Antibody (SAA0152)

    Flow Cytometry

    Flow-cytometry using anti-human CA9 antibody. Human U-87 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CA9 antibody monoclonal antibody (Catalog # HS840107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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