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Anti-EV71 VP3/Capsid protein VP3 Polyclonal Antibody (VK788014)

Anti-EV71 VP3/Capsid protein VP3 Polyclonal Antibody
Anti-EV71 VP3/Capsid protein VP3 Polyclonal Antibody
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  • 100ug
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Overview
Catalog No.VK788014
Species reactivityHuman enterovirus 71 (EV71) (EV-71)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant EV71 VP3/Capsid protein VP3 (Gly324-Gln565).
Target Genome polyprotein, P1, Capsid protein VP3, P1C, Virion protein 3
Purification Purified by antigen affinity column.
Accession B9VUU3
RRID Anti-EV71 VP3/Capsid protein VP3 Polyclonal Antibody (abinScience Cat# VK788014, RRID:AB_3723681)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Genome polyprotein (VP3/Capsid protein VP3) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization.

Note For research use only.
Images
  • Anti-EV71 VP3/Capsid protein VP3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-EV71 VP3/Capsid protein VP3 polyclonal antibody at 1ug/mL on recombinant EV71 VP3/Capsid protein VP3 (Catalog No. VK788012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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