ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Papaya ringspot virus
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
19.84 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Genome polyprotein, P1 proteinase, 3.4.-.-, N-terminal protein, Helper component proteinase, HC-pro, 3.4.22.45, Protein P3, 6 kDa protein 1, 6K1, Cytoplasmic inclusion protein, CI, 3.6.4.-, 6 kDa protein 2, 6K2, Viral genome-linked protein, VPg, Nuclear inclusion protein A, NI-a, NIa, 3.4.22.44, 49 kDa proteinase, 49 kDa-Pro, NIa-pro, Nuclear inclusion protein B, NI-b, NIb, 2.7.7.48, RNA-directed RNA polymerase, Capsid protein, CP, Coat protein
Background
Genome polyprotein is a ~381 kDa protein. Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant PRSV HC-pro Protein, N-His
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