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Recombinant DENV-1 NS3 Protein, N-His (VK694032)

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Recombinant DENV-1 NS3 Protein, N-His

Recombinant DENV-1 NS3 Protein, N-His

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Datasheet

Overview

Catalog No.	VK694032
Description	Recombinant DENV-1 NS3 Protein, N-His (VK694032) expressed in E. coli, spanning Leu1479-Lys1644. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P17763
Protein length	Leu1479-Lys1644
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Dengue virus type 1 (DENV-1)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.11 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Serine protease NS3, EC:3.4.21.91, EC:3.6.1.15, EC:3.6.4.13, Flavivirin protease NS3 catalytic subunit, Non-structural protein 3
Background	Genome polyprotein is a ~378 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions. 1. Kuhn, RJ. et al. (2002) Cell 108, 717-25. PMID: 11893341 2. Sangiambut, S. et al. (2008) The Journal of general virology 89, 1254-1264. PMID: 18420804 3. Colpitts, TM. et al. (2011) PloS one 6, e24365. PMID: 21909430 4. Bhuvanakantham, R. et al. (2013) Cellular microbiology 15, 1688-706. PMID: 23522008
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant DENV-1 NS3 Protein, N-His

References

Datasheet

Recombinant DENV-1 NS3 Protein, N-His.pdf

Formula

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Recombinant DENV-1 NS3 Protein, N-His