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Recombinant CSFV Glycoprotein E2/gp55 Protein, N-His (VK750031)

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Recombinant CSFV Glycoprotein E2/gp55 Protein, N-His

Recombinant CSFV Glycoprotein E2/gp55 Protein, N-His

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Overview
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Datasheet

Overview

Catalog No.	VK750031
Description	Recombinant CSFV Glycoprotein E2/gp55 Protein, N-His (VK750031) expressed in Insect Cells, spanning Ala739-Ala1029. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Insect Cells
Accession	P21530
Protein length	Ala739-Ala1029
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Classical swine fever virus (strain Brescia) (CSFV) (Hog cholera virus)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	41.62 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Genome polyprotein, Envelope glycoprotein E2, gp55
Background	Genome polyprotein (Glycoprotein E2/gp55) is a ~438 kDa protein. Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant CSFV Glycoprotein E2/gp55 Protein, N-His

References

Datasheet

Recombinant CSFV Glycoprotein E2/gp55 Protein, N-His.pdf

Formula

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Working Solution

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Recombinant CSFV Glycoprotein E2/gp55 Protein, N-His