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Recombinant Human JAK1 Protein, C-His (HB829022)

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Recombinant Human JAK1 Protein, C-His

Recombinant Human JAK1 Protein, C-His

Notice · Catalog HB829022 has been discontinued.

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Overview

Catalog No.	HB829022
Description	Recombinant Human JAK1 Protein, C-His (HB829022) expressed in E. coli, spanning Met1-Lys1154. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P23458
Protein length	Met1-Lys1154
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	JAK1B, JAK1, Janus kinase 1, Tyrosine-protein kinase JAK1, JAK1A, JAK-1
Background	Tyrosine-protein kinase JAK1 is a ~133 kDa protein. Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Kinase partner for the type I interferon receptor IFNAR2. In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins. Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors. 1. Staerk, J. et al. (2005) The Journal of biological chemistry 280, 41893-9. PMID: 16239216 2. Del Bel, KL. et al. (2017) The Journal of allergy and clinical immunology 139, 2016-2020.e5. PMID: 28111307 3. Gruber, CN. et al. (2020) Immunity 53, 672-684.e11. PMID: 32750333 4. Sakatsume, M. et al. (1995) The Journal of biological chemistry 270, 17528-34. PMID: 7615558 5. Müller, M. et al. (1993) Nature 366, 129-35. PMID: 8232552 6. Simoncic, PD. et al. (2002) Current biology : CB 12, 446-53. PMID: 11909529 7. Usacheva, A. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 169, 1302-8. PMID: 12133952 8. Novick, D. et al. (1995) Journal of leukocyte biology 57, 712-8. PMID: 7759950
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human JAK1 Protein, C-His

References

Datasheet

Recombinant Human JAK1 Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

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Volume

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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V₂ (Working Vol.)

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Recombinant Human JAK1 Protein, C-His