Recombinant Rhizopus delemar CotH3 Protein, N-His (JN863012)

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Overview

Catalog No.	JN863012
Description	Recombinant Rhizopus delemar CotH3 Protein, N-His (JN863012) expressed in E. coli, spanning Ala18-Thr578. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	I1CFE1
Protein length	Ala18-Thr578
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	64.01 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Invasin CotH3, Spore coat protein homolog 3, CotH3, RO3G_11882
Background	Invasin CotH3 is a ~65 kDa protein. Promotes invasion of host epithelial cells by adhering to receptors on the host cell surface to facilitate endocytosis of the pathogen into host cells. Binds HSPA5/BiP protein on the cell surface of host nasal epithelial cells. 1. Gebremariam, T. et al. (2014) The Journal of clinical investigation 124, 237-50. PMID: 24355926 2. Alqarihi, A. et al. (2020) mBio 11. PMID: 32487760
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Rhizopus delemar CotH3 Protein, N-His

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Datasheet

Recombinant Rhizopus delemar CotH3 Protein, N-His.pdf

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Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Working Solution

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Recombinant Rhizopus delemar CotH3 Protein, N-His (JN863012)

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