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Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His (JN868032)

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Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His

Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His

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Datasheet

Overview

Catalog No.	JN868032
Description	Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His (JN868032) expressed in E. coli, spanning Arg72-Gly548. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P11311
Protein length	Arg72-Gly548
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	54.04 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Adhesin P1, Attachment protein, Cytadhesin P1, mgpA, MPN_141, MP013
Background	Adhesin P1 (mgpA) is a ~176 kDa protein. Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium and plays a major role in gliding motility. This protein and P40/P90 mutually stabilize each other. The 3 adhesin proteins are required for formation of the attachment organelle. 1. Vizarraga, D. et al. (2020) Nature communications 11, 5188. PMID: 33057023 2. Vizarraga, D. et al. (2025) PLoS pathogens 21, e1012973. PMID: 40153444 3. Waldo, RH. et al. (2006) Journal of bacteriology 188, 569-75. PMID: 16385047
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His

References

Datasheet

Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His.pdf

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Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His