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Recombinant Neisseria meningitidis NHBA Protein, N-His (JN891012)

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Recombinant Neisseria meningitidis NHBA Protein, N-His

Recombinant Neisseria meningitidis NHBA Protein, N-His

Recombinant Neisseria meningitidis NHBA Protein, N-His

Recombinant Neisseria meningitidis NHBA Protein, N-His

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Overview
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References
Datasheet

Overview

Catalog No.	JN891012
Description	Recombinant Neisseria meningitidis NHBA Protein, N-His (JN891012) expressed in E. coli, spanning Met312-Asp427. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9JPP1
Protein length	Met312-Asp427
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Neisseria meningitidis
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	14.49 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Neisserial heparin binding antigen, NHBA, Genome-derived Neisseria antigen 2132, GNA2132, C2 fragment, nhba, gna2132
Background	Neisserial heparin binding antigen (NHBA/Dextranase) is a ~44 kDa protein. A major human immunogenic protein detected in patients recovering from meningitidis, where it induces bactericidal antibodies. Binds human cells, heparin and heparan sulfate proteoglycan in vitro via the Arg-rich motif. Heparin-binding to this protein protects bacteria against killing by bactericidal antibodies (serum killing). The bacteria binds a number of human extracellular sialyated and/or sulfated glycans via this protein, including chondroitin sulfate, heparin and ganglioside GT3. Binds DNA non-specifically. 1. Maritan, M. et al. (2018) PloS one 13, e0201922. PMID: 30133484
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Neisseria meningitidis NHBA Protein, N-His
Western blot

Detected by Anti-NHBA monoclonal antibody (Catalog No: JN891033)

References

JN891012-SDS-PAGE-1.jpg

Datasheet

Recombinant Neisseria meningitidis NHBA Protein, N-His.pdf

Formula

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Recombinant Neisseria meningitidis NHBA Protein, N-His