Recombinant Hendra virus Glycoprotein G Protein, N-strep (VK685011)

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Datasheet

Overview

Catalog No.	VK685011
Description	Recombinant Hendra virus Glycoprotein G Protein, N-strep (VK685011) expressed in Mammalian Cells, spanning Asn72-Ser604. Purity: >90% by SDS-PAGE. Highlights ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	O89343
Protein length	Asn72-Ser604
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Hendra virus (isolate Horse/Autralia/Hendra/1994) (HeV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	63.21 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Glycoprotein G, G
Background	Glycoprotein G is a ~67 kDa protein. Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of glycoprotein G to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Hendra virus Glycoprotein G Protein, N-strep
Bioactivity

Detects HeV/NiV Glycoprotein G Antibody (m102.4) in indirect ELISAs.
Bioactivity

Detects HeV/NiV Glycoprotein G (m102.3) in indirect ELISAs.
Western blot

Detected by Anti-Hendra virus glycoprotein G monoclonal antibody (Catalog No: VK685073)
Western blot

Detected by Anti-Hendra virus glycoprotein G monoclonal antibody (Catalog No: VK685113)
Western blot

Detected by Anti-Hendra virus glycoprotein G monoclonal antibody (Catalog No: VK685063)
Western blot

Western blot analysis of recombinant Hendra virus Glycoprotein G protein using anti-Hendra virus (HeV) Glycoprotein G monoclonal antibody (Catalog No: VK685015) at 1 µg/mL.
Lane 1: Recombinant Hendra virus Glycoprotein G Protein (Catalog No: VK685011)
Western blot

Western blot analysis of recombinant Hendra virus Glycoprotein G protein using anti-Hendra virus (HeV) Glycoprotein G monoclonal antibody (Catalog No: VK685025) at 1 µg/mL.
Lane 1: Recombinant Hendra virus Glycoprotein G Protein (Catalog No: VK685011)
Western blot

Western blot analysis of recombinant Hendra virus Glycoprotein G protein using anti-Hendra virus (HeV) Glycoprotein G monoclonal antibody (Catalog No: VK685035) at 1 µg/mL.
Lane 1: Recombinant Hendra virus Glycoprotein G Protein (Catalog No: VK685011)
Bioactivity

Detected by anti-HeV/NiV Glycoprotein G antibody (1A050) (Catalog No: VK685015) in indirect ELISA.
Bioactivity

Detected by anti-Hendra virus/HeV Glycoprotein G Monoclonal antibody (1A206) (Catalog No: VK685025) in indirect ELISA.
Bioactivity

Detected by anti-Hendra virus/HeV Glycoprotein G Monoclonal antibody (1A207) (Catalog No: VK685035) in indirect ELISA.
Bioactivity

Detected by HENV-18 (Catalog No: VK685093) in indirect ELISA.
Bioactivity

Detected by HENV-117 (Catalog No: VK685020) in indirect ELISA.
Bioactivity

Detected by HENV-1 (Catalog No: VK685053) in indirect ELISA.
Bioactivity

Detected by HENV-2 (Catalog No: VK685063) in indirect ELISA.
Bioactivity

Detected by HENV-10 (Catalog No: VK685083) in indirect ELISA.
Bioactivity

Detected by HENV-9 (Catalog No: VK685073) in indirect ELISA.
Bioactivity

Detected by HENV-21 (Catalog No: VK685103) in indirect ELISA.
Bioactivity

Detected by HENV-43 (Catalog No: VK685113) in indirect ELISA.