Recombinant HRSV G/Major surface glycoprotein G Protein, C-His (VK562011)

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Overview

Catalog No.	VK562011
Description	Recombinant HRSV G/Major surface glycoprotein G Protein, C-His (VK562011) expressed in Mammalian Cells, spanning Asn66-Gln298. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P20895
Protein length	Asn66-Gln298
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human respiratory syncytial virus A (strain Long)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.35 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Major surface glycoprotein G, Attachment glycoprotein G, Membrane-bound glycoprotein, mG, Mature secreted glycoprotein G, Mature sG, G
Background	Major surface glycoprotein G is a ~32 kDa protein. Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities.
Note	For research use only.

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Bioactivity

Detects HRSV G/Major surface glycoprotein G Antibody (CB002.5) in indirect ELISAs.
Bioactivity

Detected by Anti-HRSV-A/B G/Major surface glycoprotein G Antibody (131-2G) (Cat No.: VK562033) in indirect ELISA.
Bioactivity

Detected by InVivoMAb Anti-HRSV G/Major surface glycoprotein G Antibody (3G12) (Catalog No: VK562030) in indirect ELISA

References

Datasheet

Recombinant HRSV G/Major surface glycoprotein G Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Working Solution

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Recombinant HRSV G/Major surface glycoprotein G Protein, C-His (VK562011)

Overview

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References

Datasheet

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