Recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Protein, N-His (JN963012)

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100ug
1mg

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Datasheet

Overview

Catalog No.	JN963012
Description	Recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Protein, N-His (JN963012) expressed in E. coli, spanning Asp30-Asn500. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0C2E9
Protein length	Asp30-Asn500
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Clostridium perfringens (strain 13 / Type A)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	54.87 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163
Background	Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Protein, N-His
Bioactivity

Detects HS1 in indirect ELISAs.
Western blot

Detected by Anti-Theta-toxin monoclonal antibody (Catalog No: JN963033)
Bioactivity

Detected by Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS2) (Catalog No: JN963013) in indirect ELISA
Bioactivity

Detected by Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3) (Catalog No: JN963023) in indirect ELISA