Recombinant HBV S/L-HBsAg/L glycoprotein Protein, C-S Tag (VK539011)

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100ug
1mg

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Datasheet

Overview

Catalog No.	VK539011
Description	Recombinant HBV S/L-HBsAg/L glycoprotein Protein, C-S Tag (VK539011) expressed in Mammalian Cells, spanning Met175-Ile400. Purity: >90% by SDS-PAGE. Highlights ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	Q9E6S4
Protein length	Met175-Ile400
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Hepatitis B virus
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.41 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Large envelope protein, L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen, S
Background	Large envelope protein (HBsAg) is a ~43 kDa protein. The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant HBV S/L-HBsAg/L glycoprotein Protein, C-S Tag
Bioactivity

Detects HBV HBsAg antibody in indirect ELISAs.
Bioactivity

Detects Exbivirumab in indirect ELISAs.
Bioactivity

Detects HBV HBsAg antibody in indirect ELISAs.
Bioactivity

Detects Libivirumab in indirect ELISAs.

References

VK539011-SDS-PAGE-1.jpg

Datasheet

Recombinant HBV S/L-HBsAg/L glycoprotein Protein, C-S Tag.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Recombinant HBV S/L-HBsAg/L glycoprotein Protein, C-S Tag (VK539011)

Overview

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References

Datasheet

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