Recombinant Human SNCA Protein, N-His (HW755012)

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Datasheet

Overview

Catalog No.	HW755012
Description	Recombinant Human SNCA Protein, N-His (HW755012) expressed in E. coli, spanning Met1-Ala140. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P37840
Protein length	Met1-Ala140
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.75 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Alpha-synuclein, PARK1, Non-A4 component of amyloid precursor, NACP, Non-A beta component of AD amyloid, SNCA
Background	Alpha-synuclein (SNCA) is a ~14 kDa protein. Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. 1. Burré, J. et al. (2010) Science (New York, N.Y.) 329, 1663-7. PMID: 20798282 2. Butler, B. et al. (2015) The Journal of biological chemistry 290, 29542-54. PMID: 26442590 3. Logan, T. et al. (2017) Nature neuroscience 20, 681-689. PMID: 28288128 4. Huang, CC. et al. (2018) Journal of cell science 131. PMID: 30404828
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human SNCA Protein, N-His
Bioactivity

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Bioactivity

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Bioactivity

Detects Prasinezumab in indirect ELISAs.
Bioactivity

Detects MEDI1341 in indirect ELISAs.
Western blot

Detected by Anti-SNCA monoclonal antibody (Catalog No: HW755086)
Western blot

Detected by Anti-SNCA monoclonal antibody (Catalog No: YP661013)
Western blot

Detected by Anti-SNCA monoclonal antibody (Catalog No: HW755020)
Western blot

Detected by Anti-SNCA monoclonal antibody (Catalog No: HY524010)
Western blot

Detected by Anti-SNCA monoclonal antibody (Catalog No: HW755016)
Western blot

Detected by Anti-SNCA monoclonal antibody (Catalog No: HW755026)
Bioactivity

Detected by Research Grade Anti-Human SNCA/Alpha-synuclein (Lu AF82422) (Catalog No: HW755086) in indirect ELISA