Catalog No. | JN842012 |
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Description | Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins |
Expression system | E. coli |
Protein length | PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli. |
Applications | Removal of high mannose N-glycans from glycoproteins |
Species | Elizabethkingia miricola |
Predicted molecular weight | 37.08 kDa |
Nature | Recombinant |
Endotoxin level | Please contact with the lab for this information. |
Purity | >95% as determined by SDS-PAGE. |
Form | Liquid |
Storage buffer | 20mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol |
Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
Note | For research use only. |
SDS-PAGE for PNGase F
The deglycosylation of protein detect by SDS-PAGE
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