Research Grade Tusamitamab (HY546066)

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Spec:

100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.	HY546066
Description	Tusamitamab (HY546066) is a research-grade recombinant antibody targeting CD66. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Chimeric
Isotype	IgG1-Kappa
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	Meconium antigen 100, CEACAM5, CEA, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P06731
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	SAR-408377, 2349294-95-5
Background	Cell adhesion molecule CEACAM5 (CD66e) is a ~76 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells. 1. Taheri, M. et al. (2000) The Journal of biological chemistry 275, 26935-43. PMID: 10864933 2. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050 3. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

SEC-HPLC detection for Research Grade Tusamitamab.
Flow cytometry

Flow-cytometry using APC anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD66e monoclonal antibody (Catalog HY546066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD66e monoclonal antibody (Catalog HY546066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD66e monoclonal antibody (Catalog HY546066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD66e monoclonal antibody (Catalog HY546066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.