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Research Grade Emapalumab (HF813016)

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Research Grade Emapalumab

Research Grade Emapalumab

Research Grade Emapalumab

Research Grade Emapalumab

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100ug
1mg

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Overview
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References
Datasheet

Overview

Catalog No.	HF813016
Description	Emapalumab (HF813016) is a research-grade recombinant antibody targeting Interferon gamma. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-lambda
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	Interferon gamma, IFN-gamma, Immune interferon, IFNG
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01579
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	NI-0501, emapalumab-lzsg, 1709815-23-5
Background	Interferon gamma (IFNG/IFN-gamma) is a ~19 kDa protein. Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. 1. El Bougrini, J. et al. (2006) Cellular and molecular biology (Noisy-le-Grand, France) 52, 9-15. PMID: 16914093 2. Hisamatsu, H. et al. (1996) The Journal of experimental medicine 183, 1807-16. PMID: 8666937 3. Greenlund, AC. et al. (1993) The Journal of biological chemistry 268, 18103-10. PMID: 8349687 4. Akiyama, K. et al. (1994) FEBS letters 343, 85-8. PMID: 8163024 5. Fabunmi, RP. et al. (2001) Journal of cell science 114, 29-36. PMID: 11112687 6. Lah, TT. et al. (1995) FEBS letters 363, 85-9. PMID: 7729559
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Emapalumab.
Western blot

Western blot analysis was performed using anti-IFNG/IFN-gamma monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IFNG/IFN-gamma (Catalog No: HF813012), 50ng
Lane 2: recombinant human IFNG/IFN-gamma (Catalog No: HF813012), 30ng
Lane 3: recombinant human IFNG/IFN-gamma (Catalog No: HF813012), 20ng

References

Datasheet

Research Grade Emapalumab.pdf

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Research Grade Emapalumab