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Research Grade Necitumumab (HF004016)

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  • 100ug
  • 1mg
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Overview
Catalog No.HF004016
Species reactivityHuman
ApplicationsResearch Grade Biosimilar
Host speciesHuman
IsotypeIgG1-kappa
Expression systemMammalian Cells
ClonalityMonoclonal
TargetHER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
Endotoxin levelPlease contact the lab for this information.
Purity>95% purity as determined by SDS-PAGE.
PurificationProtein A/G, purified from cell culture supernatant.
AccessionP00533
FormLiquid
Storage buffer0.01M PBS,pH7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names11F8,IMC-11F8,LY3012211,CAS:906805-06-9
BackgroundNecitumumab is a human IgG monoclonal antibody directed against EGFR. Necitumumab can be used for research of NSCLC, colorectal cancer, etc.
NoteFor research use only. Not for use in clinical or therapeutic applications.
Images
  • SEC-HPLC

    SEC-HPLC detection for Research Grade Necitumumab.

  • Bioactivity

    Detects Human EGFR/ERBB1/HER1 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human EGFR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFR monoclonal antibody (Catalog HF004016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Research Grade Necitumumab.

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