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Research Grade Bevacizumab (HB941026)

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  • 100ug
  • 1mg
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Overview
Catalog No.HB941026
Species reactivityHuman
ApplicationsResearch Grade Biosimilar
Host speciesHumanized
IsotypeIgG1-kappa
Expression systemMammalian Cells
ClonalityMonoclonal
TargetVascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A
Endotoxin levelPlease contact the lab for this information.
Purity>95% purity as determined by SDS-PAGE.
PurificationProtein A/G, purified from cell culture supernatant.
AccessionP15692
FormLiquid
Storage buffer0.01M PBS,pH7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names12-IgG1,F(ab)-12IgG1,Fab-12IgG1,rhuMAb-VEGF,ABP215,CAS:216974-75-3
BackgroundBevacizumab, a humanized IgG1 monoclonal antibody, specifically binds to all VEGF-A isoforms with high affinity.
NoteFor research use only. Not for use in clinical or therapeutic applications.
Images
  • SEC-HPLC

    SEC-HPLC detection for Research Grade Bevacizumab.

  • Bioactivity

    Detects Human VEGF165 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human VEGFA antibody. U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA monoclonal antibody (Catalog HB941026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Research Grade Bevacizumab.

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