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Research Grade Conatumumab (HT348036)

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  • 100ug
  • 1mg
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  • Datasheet
Overview
Catalog No.HT348036
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHuman
IsotypeIgG1-kappa
Expression system Mammalian Cells
Clonality Monoclonal
Target TRAILR2, TRAIL-R2, Death receptor 5, CD262, DR5, Tumor necrosis factor receptor superfamily member 10B, ZTNFR9, TRAIL receptor 2, TRICK2, TNFRSF10B, KILLER, TNF-related apoptosis-inducing ligand receptor 2
Endotoxin level Please contact the lab for this information.
Purity >95% purity as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession O14763
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate NamesAMG655, TRAIL-R2mAb, XG1-048vw, 896731-82-1
Note For research use only. Not suitable for clinical or therapeutic use.
Images
  • Bioactivity

    Detects CD262/TNFRSF10B/TRAIL-R2/DR5 in indirect ELISAs.

  • SDS-PAGE

    SDS-PAGE for Research Grade Conatumumab.

  • Flow CytoMetry

    Flow-cytometry using APC anti-human CD262 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD262 monoclonal antibody (Catalog: HT348036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human CD262 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD262 monoclonal antibody (Catalog: HT348036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using PE anti-human CD262 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD262 monoclonal antibody (Catalog: HT348036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-CD262 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human CD262 (Catalog No: HT348011)
    Lane 2: negative control

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