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InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1) (HN876010)

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InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1)

InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1)

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100ug
1mg

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Datasheet

Overview

Catalog No.	HN876010
Description	InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1) [CDB-1] (HN876010) is a human monoclonal antibody detecting Toxin B in ELISA, Neutralization.
Species reactivity	Clostridioides difficile
Applications	ELISA, Neutralization
Host species	Human
Isotype	IgG1, kappa
Clone ID	CDB-1
Clonality	Monoclonal
Target	Toxin B, 3.4.22.-, Glucosyltransferase TcdB, 2.4.1.-, tcdB, toxB, Clostridium difficile Toxin
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P18177
RRID	InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1) (abinScience Cat# HN876010, RRID:AB_3727152)
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis. 1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489 2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149 3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482 4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933 5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463 6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936 7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356 9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706
Note	For research use only. Not suitable for clinical or therapeutic use.

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SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1).

References

Datasheet

InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1).pdf

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InVivoMAb Anti-Clostridioides difficile Toxin B/tcdB Antibody (CDB-1)