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Anti-JEV Envelope protein E Antibody (2H4) (VK731023)

Anti-JEV Envelope protein E Antibody (2H4)
Anti-JEV Envelope protein E Antibody (2H4)
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  • Datasheet

Overview
Catalog No.VK731023
Description
Anti-JEV Envelope protein E Antibody (2H4) [2H4] (VK731023) is a mouse monoclonal antibody detecting Envelope protein E in ELISA, Neutralization, SPR.
Highlights
  • Multi-Application — Validated across multiple applications.
Species reactivityJapanese encephalitis virus
ApplicationsELISA, Neutralization, SPR
Host speciesMouse
IsotypeIgG2a, kappa
Clone ID2H4
Clonality Monoclonal
Target Genome polyprotein, Envelope protein E
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P32886
RRID Anti-JEV Envelope protein E Antibody (2H4) (abinScience Cat# VK731023, RRID:AB_3727133)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Genome polyprotein (Envelope protein E) is a ~380 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

Note For research use only.
Images
  • Anti-JEV Envelope protein E Antibody (2H4)

    SDS-PAGE

    SDS-PAGE for Anti-JEV Envelope protein E Antibody (2H4).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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