Anti-HPV16 L1/Major capsid protein L1 Antibody (H16.V5) (VK461053)

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Datasheet

Overview

Catalog No.

VK461053

Description

Anti-HPV16 L1/Major capsid protein L1 Antibody (H16.V5) [H16.V5] (VK461053) is a mouse monoclonal antibody detecting Major capsid protein L1 in ELISA, Neutralization, WB.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human papillomavirus type 16

Applications

ELISA, Neutralization, WB

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

H16.V5

Clonality

Monoclonal

Target

L1, Major capsid protein L1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P03101

RRID

Anti-HPV16 L1/Major capsid protein L1 Antibody (H16.V5) (abinScience Cat# VK461053, RRID:AB_3725270)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Major capsid protein L1 is a ~56 kDa protein. Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis.

Note

For research use only.

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