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Anti-Human Reticulon-4/RTN4 Antibody (SAA0170), APC (HP021137)

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Overview
Catalog No.HP021137
Description
Anti-Human Reticulon-4/RTN4 Antibody (SAA0170), APC [SAA0170] (HP021137) is a human monoclonal antibody detecting Reticulon-4 in ELISA, FCM. Suitable for Human.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
  • Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesHuman
IsotypeIgG1
Clone IDSAA0170
ConjugationAPC
Clonality Monoclonal
Target RTN-x, Reticulon-4, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, NOGO, Nogo protein, Neurite outgrowth inhibitor, NSP, Reticulon-5, KIAA0886, RTN4, Foocen
Endotoxin level Please contact with the lab for this information.
Accession Q9NQC3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Reticulon-4 (RTN4) is a ~129 kDa protein. Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities (Probable).

1. Yamamoto, Y. et al. (2014) The Biochemical journal 458, 69-79. PMID: 24262037
2. Urade, T. et al. (2014) The Kobe journal of medical sciences 60, E57-65. PMID: 25612671
3. Wang, S. et al. (2016) eLife 5. PMID: 27619977
4. Rämö, O. et al. (2016) Scientific reports 6, 35969. PMID: 27786289
5. Christodoulou, A. et al. (2016) Journal of cell science 129, 1552-65. PMID: 26906412
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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