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Anti-Human SNCA/Alpha-synuclein Antibody (D10), FITC (HW755117)

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Overview

Catalog No.	HW755117
Description	Anti-Human SNCA/Alpha-synuclein Antibody (D10), FITC [D10] (HW755117) is a mouse monoclonal antibody detecting Alpha-synuclein in ELISA, FCM, IF, WB. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	ELISA, FCM, IF, WB
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	D10
Conjugation	FITC
Clonality	Monoclonal
Target	Alpha-synuclein, PARK1, Non-A4 component of amyloid precursor, NACP, Non-A beta component of AD amyloid, SNCA
Endotoxin level	Please contact with the lab for this information.
Accession	P37840
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Alpha-synuclein (SNCA) is a ~14 kDa protein. Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. 1. Burré, J. et al. (2010) Science (New York, N.Y.) 329, 1663-7. PMID: 20798282 2. Butler, B. et al. (2015) The Journal of biological chemistry 290, 29542-54. PMID: 26442590 3. Logan, T. et al. (2017) Nature neuroscience 20, 681-689. PMID: 28288128 4. Huang, CC. et al. (2018) Journal of cell science 131. PMID: 30404828
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Human SNCA/Alpha-synuclein Antibody (D10), FITC.pdf

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Anti-Human SNCA/Alpha-synuclein Antibody (D10), FITC