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Anti-Human CD27 Antibody (5F24) (HB796207)

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Overview
Catalog No.HB796207
Species reactivityHuman
ApplicationsFCM, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone ID5F24
Clonality Monoclonal
Target CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P26842
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Bioactivity

    Detects Human CD27 in indirect ELISAs.

  • Flow CytoMetry

    Flow-cytometry using anti-human CD27 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD27 monoclonal antibody (Catalog: HB796207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-CD27 monoclonal antibody at 1µg/ml on various samples.
    Lane 1: recombinant human CD27 (Catalog No: HB796011)
    Lane 2: negative control

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