Catalog No. | HY248207 |
---|---|
Species reactivity | Human |
Applications | FCM |
Host species | Mouse |
Isotype | IgG1, kappa |
Conjugation | Unconjugated |
Clonality | Monoclonal |
Target | MSK12, VLA-4 subunit beta, CD29, Integrin beta-1, ITGB1, FNRB, MDF2, GPIIA, Glycoprotein IIa, Fibronectin receptor subunit beta |
Concentration | 1 mg/ml |
Endotoxin level | Please contact with the lab for this information. |
Purity | >95% as determined by SDS-PAGE. |
Purification | Protein A/G purified from cell culture supernatant. |
Accession | P05556 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
Note | For research use only. |
Detects Human CD29/ITGB1 in indirect ELISAs.
Flow-cytometry using anti-human CD29 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD29 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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