Anti-Human CD33 Antibody (SAA1404) (HB185307)

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Overview

Catalog No.	HB185307
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	SAA1404
Clonality	Monoclonal
Target	Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P20138
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human CD33 Antibody (ABS0480).
Flow Cytometry

Flow-cytometry using anti-human CD33 antibody.CD33 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD33 antibody.RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet