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Anti-Human CD86/B7-2 Antibody (SAA1397) (HW776207)

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Overview
Catalog No.HW776207
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA1397
ClonalityMonoclonal
TargetBU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1
Endotoxin levelPlease contact with the lab for this information.
Purity>95% as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionP42081
FormLiquid
Storage buffer0.01M PBS, pH 7.4, 0.09% Sodium azide.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
NoteFor research use only.
Images
  • Bioactivity

    Detects Human CD86/B7-2 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human CD86 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD86 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

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