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Anti-Human IL33 Antibody (SAA0395) (HF543107)

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Overview
Catalog No.HF543107
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesMouse
IsotypeIgG1, lambda
Clone IDSAA0395
ConjugationUnconjugated
ClonalityMonoclonal
TargetNF-HEV, IL-1F11, C9orf26, IL-33, Interleukin-1 family member 11, IL33, Nuclear factor from high endothelial venules, NFHEV, Interleukin-33, IL1F11
Concentration1 mg/ml
Endotoxin levelPlease contact with the lab for this information.
Purity>95% as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionO95760
FormLiquid
Storage buffer0.01M PBS, pH 7.4, 0.09% Sodium azide.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
NoteFor research use only.
Images
  • Flow-cytometry

    Flow-cytometry using anti-human IL33 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow-cytometry

    Flow-cytometry using anti-human IL33 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow-cytometry

    Flow-cytometry using anti-human IL33 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

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